SARS-CoV-2 pseudovirus enters the host cells through spike protein-CD147 in an Arf6-dependent mannerSARS-CoV-2 슈도바이러스는 Arf6 의존적 방식으로 스파이크 단백질-CD147을 통해 숙주 세포에 들어갑니다.Article Published on 2022-12-012022-09-11 Journal: Emerging Microbes & Infections [Category] COVID19(2023년), SARS, 변종, 유전자 메커니즘, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 approach Arf6 blockade CD147 cells clathrin Clathrin-mediated endocytosis clinically coronavirus endocytic endocytic mechanism endocytosis evaluate feasible function host cell host cells Huh-7 cell Huh-7 cells independent independent of indicated infected Vero E6 cells Infection inhibited inhibitor knockdown Localization macropinocytosis pathway Prevent pseudovirus pseudovirus infection public health RAB5A SARS-CoV-2 SARS-CoV-2 entry SARS-COV-2 infection SARS-CoV-2 pseudovirus SARS-CoV-2 pseudovirus infection severe acute respiratory syndrome Coronavirus Spike protein spike protein. Spread the SARS-CoV-2 variant Vero E6 VERO E6 cells viral infection weakened [DOI] 10.1080/22221751.2022.2059403 PMC 바로가기 [Article Type] Article
The Integral Membrane Protein ZMPSTE24 Protects Cells from SARS-CoV-2 Spike-Mediated Pseudovirus Infection and Syncytia FormationArticle Published on 2022-10-262022-11-15 Journal: mBio [Category] COVID19(2023년), SARS, 유전자 메커니즘, 치료제, [키워드] acute respiratory syndrome appear assays catalytic caused Cell cell-cell fusion cell-cell fusion assay cells cellular cellular component cellular membrane contribute coronavirus coronavirus coronavirus SARS-CoV-2 Coronaviruses COVID-19 COVID-19 pandemic dead defend Defense Efficiency ENhance entry of SARS-CoV-2 examined Formation genetic knockout global public health host cell membranes IFITM IFITMs in viral include Infection inhibiting inhibitory activity innate immune innate immune response Integral interact Interaction interfere intrinsic knockdown lentivirus maturation mechanism membrane membrane protein MHV mouse hepatitis virus nuclear overexpression pandemic Plaque assay precursor prelamin A Prevent protease Proteases PROTECT Protective protective role Protein proteolytic proteolytic activity reduce required restriction factor restriction factor. SARS-CoV-2 SARS-CoV-2 pseudovirus infection shown spike Therapeutic benefit transmembrane protein viral infection viral infections virus virus entry viruses wild-type ZMPSTE24 [DOI] 10.1128/mbio.02543-22 PMC 바로가기
NSP4 and ORF9b of SARS-CoV-2 Induce Pro-Inflammatory Mitochondrial DNA Release in Inner Membrane-Derived VesiclesArticle Published on 2022-09-232022-11-16 Journal: Cells [Category] SARS, 유전자 메커니즘, [키워드] airway epithelial cells anti-apoptotic approach BCl2 BCL2 antagonist/killer caused cell death cell-free mitochondrial DNA changes co-culture co-cultured COVID-19 COVID-19 patient Effect Effects epithelial cells functional induce inhibit Inner Interaction intercellular intercellular mitochondrial transfer knockdown MCL1 mechanism membrane vesicle mitigate mitochondrial mitochondrial DNA Mortality MSC MSCs myeloid cell myeloid cell leukemia-1 nsp4 Orf9b outer membrane overexpression plasma pro-apoptotic pro-inflammatory Protein Release reversed SARS-CoV-2 SARS-CoV-2 protein screened significantly stem cell therapeutic Therapeutic approach these cell transfer Treatment tunneling nanotubes. [DOI] 10.3390/cells11192969 PMC 바로가기
SARS-CoV-2 Nsp14 protein associates with IMPDH2 and activates NF-κB signalingArticle Published on 2022-09-132022-11-16 Journal: Frontiers in Immunology [Category] SARS, 유전자 메커니즘, [키워드] activate Activation acute respiratory syndrome bortezomib caused contribute coronavirus COVID-19 patients cytokine Host identify IL-6 IL-8 IMPDH2 Infection inhibitor Interaction knockdown lung tissue sample mechanism mycophenolic acid NF-κB NF-κB activation NF-κB signaling nsp14 nuclear translocation occurred p65 pro-inflammatory cytokines promote Protein proteomic regulate ribavirin ribavirin. SARS-CoV-2 SARS-CoV-2 infected cells SARS-COV-2 infection the SARS-CoV-2 upregulation viral infection viral replication [DOI] 10.3389/fimmu.2022.1007089 PMC 바로가기
FACT subunit SUPT16H associates with BRD4 and contributes to silencing of interferon signalingFACT 소단위 SUPT16H는 BRD4와 연관되어 인터페론 신호 전달 억제에 기여합니다Article Published on 2022-08-262022-09-11 Journal: Nucleic Acids Research [Category] SARS, 유전자 메커니즘, [키워드] acetylated acetylation Activation Affect association BRD4 cause CBL0137 cells chromatin co-culture complex composed contribute demonstrated Depletion domain enzyme epithelial EZH2 gene transcription Genetic HDAC1 heterodimeric protein histone chaperone IFN IFN signaling IFNs infected cells Infection Influenza inhibit inhibitor interferon interferon signaling interferon-stimulated gene interferon-stimulated genes ISGs killing knockdown lysine mechanism Modification natural killer NK cells pharmacological promote promoter Protein protein stability regulate regulated reported RNAi SARS-CoV-2 shown Signaling SSRP1 subunit suppression SUPT16H Transcription treat viral infections virus-infected cell virus-infected cells viruses Zika [DOI] 10.1093/nar/gkac645 PMC 바로가기 [Article Type] Article
Cas13d knockdown of lung protease Ctsl prevents and treats SARS-CoV-2 infection Research Published on 2022-07-252022-10-05 Journal: Nature chemical biology [Category] COVID19(2023년), SARS, 변종, 치료법, 치료제, [키워드] alleviate approach can be used cathepsin L Cell CRISPR decrease expression Host host protease in vivo infected with SARS-CoV-2 inhibitor interstitial inflammation knockdown Lower lung Messenger RNA mice mRNA pathological changes Potential treatment Prevent proinflammatory protease reduced reported SARS-CoV-2 entry SARS-COV-2 infection SARS-CoV-2 pathogenesis severity survival treat Treatment unique virus [DOI] 10.1038/s41589-022-01094-4 [Article Type] Research
Social isolation reinforces aging-related behavioral inflexibility by promoting neuronal necroptosis in basolateral amygdala Research Published on 2022-07-152022-10-05 Journal: Molecular Psychiatry [Category] 유전자 메커니즘, [키워드] Activation aging application basolateral characterized cognitive correlated Critical dysfunction expression facilitated functions Genetic impairment inhibitor Intervention Isolation knockdown mechanism mice molecular mechanism mTORC1 signaling necroptosis neuronal Phenotypes prevalent promote reduction reported respond reversed risk factor social isolation wild-type [DOI] 10.1038/s41380-022-01694-5 [Article Type] Research
Characterization of a thermostable Cas13 enzyme for one-pot detection of SARS-CoV-2SARS-CoV-2의 원팟 검출을 위한 열안정성 Cas13 효소의 특성화Article Published on 2022-07-122022-09-11 Journal: Proceedings of the National Academy of Sciences of [Category] COVID19(2023년), SARS, 치료기술, [키워드] activity acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 characterization characterized clinical samples coronavirus CRISPR CRISPR Cas13 CRISPR Diagnostics CRISPR-Ca Critical cross-reactivity Data collection demonstrated develop diagnostics enzyme exhibit expand facilitate feature knockdown limit of detection mobile phone OPTIMA organism ortholog Patient Phylogenetic POC point of care power protocol qRT-PCR RNA robust SARS-CoV-2 SARS-CoV-2 detection SARS-CoV-2 genome sensitivity severe acute respiratory syndrome Coronavirus specificity temperature Therapies thermophilic bacterium thermostability thermostable Cas13 Transcriptome transcriptome editing. Type virus [DOI] 10.1073/pnas.2118260119 PMC 바로가기 [Article Type] Article
PROTACs: great opportunities for academia and industry (an update from 2020 to 2021) Reviews Published on 2022-06-092022-10-05 Journal: Signal Transduction and Targeted Therapy [Category] 치료제, [키워드] addition Cancer catalytic Chimera Clinical treatment Compound Degradation disease Diseases immune disorders increase in induce introduced knockdown molecular neurodegenerative problem Protein rapid increase reported Research researcher small molecule target target protein unique viral infections [DOI] 10.1038/s41392-022-00999-9 [Article Type] Reviews
Expanding RNAi therapeutics to extrahepatic tissues with lipophilic conjugates Research Published on 2022-06-022022-10-05 Journal: Nature biotechnology [Category] Coronavirus, 진단, [키워드] administration Alzheimer’s disease amyloid precursor protein approved Cell cell types Central nervous system CNS conjugate Efficacy evaluated hepatocyte intranasal intravitreal knockdown liver lung non-human primate organs physiological Region resulting RNA RNA interference rodent Safe siRNA siRNAs specificity sustained target gene therapeutic Therapeutics tissue [DOI] 10.1038/s41587-022-01334-x [Article Type] Research